Xialine: saliva substitute based on xanthan gum with visco-elastic properties, like sublingual mucin.

Presentatie over: "Xialine: saliva substitute based on xanthan gum with visco-elastic properties, like sublingual mucin."— Transcript van de presentatie:

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10 Xialine: saliva substitute based on xanthan gum with visco-elastic properties, like sublingual mucin

11 peptide SRCRF2: GRVEVLYRGSW SRCR Domain of agglutinin Peptide SRCRF2 endows salivary agglutinin with its binding and aggregating properties  -turn

12 Synthetic peptides bovine Lactoferrin - LFampin 268-284 (red) WKLLSKAQEKFGKNKSR - LFampin 265-284 (red+green) DLIWKLLSKAQEKFGKNKSR lactoferrampin

13 LFampin 265-284 (red+green) Control

14 Protease remmers in speeksel: cystatinen Drie functionele domeinen in cystatine Binding van cystatine aan een protease

15 TEETH FOOD Remineralisation Buffering Digestion Taste Bolus Anti-viral Lubrication, viscoelasticity Anti- bacterial Anti- fungal Inhibition of demineralization Peptides Functions of saliva M ICROBES Peptides

16 Schimmel/gist infectie: Candidiasis Onvoldoende bescherming door speeksel Candida albicans

17 ANTIMICROBIELE PEPTIDEN KARAKERISTIEKE EIGENSCHAPPEN - kleine eiwitten, 1-5 kDa - positief geladen domein - neiging tot vorming van amphipathische structuren: - hydrofobe en hydrofiele kant - affiniteit voor hydrofiele-hydrofobe interfaces - affiniteit voor negatief geladen membranen + + +

18 Candida albicans in hyphal form Control After incubation with histatin 5