Vaccinatie tegen Hepatitis B virus ELISA: Primaire en secundaire antistofrespons Edwin Tijhaar.

Presentatie over: "Vaccinatie tegen Hepatitis B virus ELISA: Primaire en secundaire antistofrespons Edwin Tijhaar."— Transcript van de presentatie:

1 Vaccinatie tegen Hepatitis B virus ELISA: Primaire en secundaire antistofrespons
Edwin Tijhaar

2 Antistoffen worden door B cellen gemaakt
Specifieke immuniteit B cel receptor bindt antigeen Geactiveerde B cel gaat sterk delen Antistoffen (= extracelluaire deel B cel receptor) worden uitgescheiden Figure 1-1 Innate and adaptive immunity. The mechanisms of innate immunity provide the initial defense against infections. Adaptive immune responses develop later and consist of activation of lymphocytes. The kinetics of the innate and adaptive immune responses are approximations and may vary in different infections.

3 Specifieke antistof productie berust of selectie
Heel veel verschillende B cellen in beenmerg gemaakt Elke B cel heeft eigen unieke receptor (antistof) Selectie van specifieke B cell Activatie en deling Productie antilichaam Memory cells Th Figure 1-7 The clonal selection hypothesis. Each antigen (X or Y) selects a preexisting clone of specific lymphocytes and stimulates the proliferation and differentiation of that clone. The diagram shows only B lymphocytes giving rise to antibody-secreting effector cells, but the same principle applies to T lymphocytes. IgG Y IgM Y

4 Primaire and secundaire immuunreactie
Secondaire immuunreactie: Sneller Sterker Beter (sterker bindende antistoffen) Door groter aantal aantal specifieke (geheugen of “memory”) cellen. Figure 1-4 Specificity, memory, and contraction of adaptive immune responses. Antigens X and Y induce the production of different antibodies (specificity). The secondary response to antigen X is more rapid and larger than the primary response (memory). Antibody levels decline with time after each immunization (contraction, the process that maintains homeostasis). The same features are seen in cell-mediated immune responses.

5 (anti-IgM of anti-IgG met HRP)
Practicum: Hepatitis B virus vaccinatie 1 2 3 4 5 6 7 8 9 10 11 weken na eerste HBV vaccinatie HBV ELISA: bepalen HBV antistoffen Voor elke week serummonster Bepaal hierin HBV (HBsAg) specifieke antistoffen Onderscheid IgM en IgG kleurloos blauwgroen Secundaire antistof (anti-IgM of anti-IgG met HRP) Primaire antistof (serummonsters) Stap 1 en 2 zijn al uitgevoerd. Figure 1-4 Specificity, memory, and contraction of adaptive immune responses. Antigens X and Y induce the production of different antibodies (specificity). The secondary response to antigen X is more rapid and larger than the primary response (memory). Antibody levels decline with time after each immunization (contraction, the process that maintains homeostasis). The same features are seen in cell-mediated immune responses.

6 Succes!

7 Practicum: Hepatitis B virus vaccinatie
1 2 3 4 5 6 7 8 9 10 11 weken na eerste HBV vaccinatie HBV ELISA: bepalen HBV antistoffen Voor elke week serummonster Bepaal hierin HBV (HBsAg) specifieke antistoffen Onderscheid IgM en IgG Figure 1-4 Specificity, memory, and contraction of adaptive immune responses. Antigens X and Y induce the production of different antibodies (specificity). The secondary response to antigen X is more rapid and larger than the primary response (memory). Antibody levels decline with time after each immunization (contraction, the process that maintains homeostasis). The same features are seen in cell-mediated immune responses.

8 Nabespreking weken na eerste HBV vaccinatie IgG IgG IgM Vragen
1 2 3 4 5 6 7 8 9 10 11 weken na eerste HBV vaccinatie HBV Vragen Welke lijn geeft de IgG respons weer? Welke grafiek geeft het verloop van IgM en IgG juist weer? kleuring HBV Figure 1-4 Specificity, memory, and contraction of adaptive immune responses. Antigens X and Y induce the production of different antibodies (specificity). The secondary response to antigen X is more rapid and larger than the primary response (memory). Antibody levels decline with time after each immunization (contraction, the process that maintains homeostasis). The same features are seen in cell-mediated immune responses. Memory cells Th HBV IgG Y IgG Y weken na eerste HBV vaccinatie IgM Y weken na eerste HBV vaccinatie