BIO-INFORMATICA OEFENZITTING 1: INFORMATION RESOURCES Deel I: Informatie over een menselijk gen Deel II: Het vinden van homologen m.b.v. BLAST Deel III. Het vinden van motieven in proteïnen Deel IV: Multiple Sequence Alignment
INTRODUCTION: FROM CHROMOSOME TO PROTEIN
Each human cell: 46 chromosomes (2x22 + 2)
Each chromosome: one long DNA double strand
SPLICING: INTRON EXCISION
Start codon AUG … Stopcodon: CDS: coding sequence
Transcriptional regulation: binding of transcription factors
Complex transcriptional initiation complex: Multiple binding motifs involved
Various gene variants may occur: share common ancestral gene in evolution “Homologous proteins” or “Protein neighbors”
Blast: searching for homologous sequences
ClustalW: multiple sequence alignment